Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------------------------------------MISNQQQKDLKK-RAAFKKLNVAMNSYVELLFLSVPLIHIFKWLG-----SLALHLIH-------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4CI0 Chain:A ((2-386)) SERIVISPTSRQEGHAELVMEVDDEGIVTKGRYFSITPVRGLEKMVTGKAPETAPVMVQRICGVCPIPHTLASVEAIDDSLDIEVPKAGRLLRELTLAAHHVNSHAIHHFLIAPDFVPENLMADAINSVSEIRKNAQYVVDMVAGEGIHPSDVRIGGMADNITELARKRLYARLKQLKPKVNEHVELMIGLIEDKGLPEGLGVHNQPTLASHQIYGDRTKFDLDRFTEIMPESWYDDPEIAKRACSTIPLYDGRNVEVGPRARMVEFQGFKERGVVAQHVARALEMKTALSRAIEILDELDTSAPVRADFDERGTGKLGIGAIEAPRGLDVHMAKVENGKIQFYSALVPTTWNIPTMGPATEGFHHEYGPHVIRAYDPCLSCATH

General information: TITO was launched using: RESULT: Template: 4CI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 16 780 48.75 15.00 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 48.75 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.126

(partial model without unconserved sides chains): PDB file : Tito_4CI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CI0-query.scw PDB file : Tito_Scwrl_4CI0.pdb: