Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGYLVLEDGTSFSGELDGHENCTGEAVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNYGINEKDFESKKPQVKAAVVYEACDHFSHYEAVYSLKEYLQKWNIPLLTHVDTRAVVKKIRANGTMGATVTASKEGAEIALQPENVAEQASAQEISTFGDGNKH-IALIDFG--YKKSIASSLVKRGCKVTVVPYQQMEA-VYNIKPDGIVLSNGP------GDPKAIQPYLGKIKSIISRFPTLGICLGHQLIALAFGGNTFKLPFGHRGANHPVIDRKTKRVFMTSQNHSYVVDEQSINEEELTIRFHHVNDTSVEGLAHKKLPVMSVQFHPEAHPGPAESEWIFDDYLKNVIPARREIAHA 1GPM Chain:A ((6-122)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------HKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEAQIRDFNPSGIILSGGPESTTEENSPRAPQ-YVFE-----AGVPVFGVCYGMQTMAMQLGGHV------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 359 -59272 -165.10 -697.31 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -165.10 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_1GPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GPM-query.scw PDB file : Tito_Scwrl_1GPM.pdb: