Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARKKKKKHEDEHVDESWLVPYADILTLLLALFIVLYASSSIDAAKFQMLSKSFNEVFTGGTGVLDYSSVTPPENESDGIDEVKKEKEEKEKNKKEKEKAADQEELENVKSQVEKFI----KDKKLEHQLETKMTSEGLLITIKDS---IFFDSGKATIRKEDVPLAKEISNLLVINP-----PRNIIISGHTDNMPIKNSE-FQSNWHLSVMRAVNFMGLLIENPKLDAKVFSAKGYGEYKPVASNKTAEGRSKNRRVEVLILPRGAAETNEK 2ZVY Chain:A ((99-273)) ----------------------------------------------------------------------------------------EKQPNIDELKKRMEQSRLNKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPMFKTGSAEVE----PYMRDI--LRAIAPVLNGIPNRISLAGHTDDFPYANGEKGYSNWELSADRANASRRELVAGGLDNGKVLRVVGMAATMRLSDRGPDD--AINRRISLLVLNKQAEQA---

General information: TITO was launched using: RESULT: Template: 2ZVY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 712 -44059 -61.88 -271.97 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -61.88 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_2ZVY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZVY-query.scw PDB file : Tito_Scwrl_2ZVY.pdb: