Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTRKESELEGVTLLGNQGTNYLFEYAPDVLESFPNKHVNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPDEKMVESKSLKLYLFSFRNHGDFHEDCMNIIMNDLIELMDPRYIEVWGKFTPRGGISIDPYTNYGKPGTKYEKMAEYRMMNHDLYPETIDNR 4FGC Chain:C ((21-165)) --------------------NYLFEYAPDVLESFPNKHVNRDYFVKFNCPEFTSLAPKTGQPDFATIYISYIPDEKMVESKSLKLYLFSFRNHGDFHEDCMNIIMNDLIELMDPRYIEVWGKFTPRGGISIDPYTNYGKPGTKYEKMAEYRMMNHDLYPETIDNR

General information: TITO was launched using: RESULT: Template: 4FGC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 612 -72929 -119.17 -502.96 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.95 3D Compatibility (PKB) : -119.17 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_4FGC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FGC-query.scw PDB file : Tito_Scwrl_4FGC.pdb: