Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MSQSNYRPSVPRW-VGDILELDKKRRQNQYRGSLTSGQEKKDWDEWKRRYSRKLKYARLNGWTIEEE------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4Q0C Chain:A ((33-545)) SQELTLVGKAAVPDVEVALDGDDWRWLARKRVLTLGVYAPDIPPFDVTYGERYEGLTADYMAIIAHNLGMQAKVLRYPTREQALSALESGQIDLIGTVNGTDGRQQSLRLSVPYAADHPVIVMPIGARHVPASNLAGQRLAVDINYLPKETLARAYPQATLHYFPSSEQALAAVAYGQADVFIGDALTTSHLVSQSYFNDVRVVAPAHIATGGESFGVRADNTRLLRVVNAVLEAIPPSEHRSLIYRWGLGSSISLDFAHPAYS-----------AREQQWMADHP-VVKVAVLNLFAPFTLFRTDEQFGGISAAVLQLLQLRTGLDFEIIGVDTVEELIAKLRSGEADMAGALFVNSARESFLSFSRPYVRNGMVIVTRQDPDAPVDADHLDGRTVALVRNSAAIPLLQRRYPQAKVVTADNPSEAMLMVANGQADAVVQTQISASYYVNRYFAGKLRIASALDLPPAEIALATTRGQTELMSILNKALYSISNDELASIISRWRGSDGDPRTWYAYRNEIYLE

General information: TITO was launched using: RESULT: Template: 4Q0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 71 5962 83.96 110.40 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.50 3D Compatibility (PKB) : 83.96 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_4Q0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q0C-query.scw PDB file : Tito_Scwrl_4Q0C.pdb: