Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------MNELKDFFFLGKPIQTEIGEIDFIRLKDYPLYTKELSMLRMNKKSLIKEY--SRFNEDGSLDPFIIEMKKRDLYEIVHSVLPDFHEAYFKVFSKVLINKDSLSLIGKHNFPRLRKLILDMHCIT----------EDKVVDNDE---------------------LQEFHDISKSLKQ-----------QDSQSDLKDIVSCVAAFNGY----TYEEISEMTMYQLYLS----FYRMAEVMNYNTTTLFATVSPDVK--VSDWSSHINLYKEESYHLSTKDAKNIEQLFGG-- 2GT1 Chain:A ((1-323)) MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQIPSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAALVTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQ-----QHAVERTRELFAKSLGYSKPQTQGDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWGAPHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNITVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKA

General information: TITO was launched using: RESULT: Template: 2GT1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1016 37148 36.56 160.81 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 36.56 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.203

(partial model without unconserved sides chains): PDB file : Tito_2GT1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GT1-query.scw PDB file : Tito_Scwrl_2GT1.pdb: