Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDVKKNENMTFEEAMKGLESIVSKLEE---GDVPLEQAINYFQEGMALSKMCHEKLQKVEKQMDFILKEDGELAPFSVQEEDEGDK 3MXZ Chain:A ((22-87)) ------KELHSYEKEVEREAAKTADMKDKGADPYDLKQQENVLGESRMMIPDCHKRLESALADLKSTLAEL----------------

General information: TITO was launched using: RESULT: Template: 3MXZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 97 -1922 -19.81 -31.00 target 2D structure prediction score : 0.84 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -19.81 2D Compatibility (Sec. Struct. Predict.) : 0.84 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_3MXZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MXZ-query.scw PDB file : Tito_Scwrl_3MXZ.pdb: