Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEA-LPAGEQFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIAHIS--DEALRSGLVKAAWPGRLELVQE-HPPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPYQNMIKRLETIAHAIHFASFDF---PRASLAKDLYD---ASEISNKSWSEDPDDVIKFIESKKGSNEIVLITGSLYFISDIRKRLK 1O5Z Chain:A ((12-431)) -HMAYLEVLRYLYHK----VKPGLERISMLLSKLGNPHLEYKTIHIGGTNGKGSVANMVSNILVSQGYRVGSYYSPHLSTFRERIRLNEEYISEEDVVKIYETMEPILNELDKEEIFSPSFFEVVTAMAFLYFAEK-NVDIAVLEVGLGGRLDATNVVFPLCSTIVTVDR-------YTIEQIAWEKSGIIKERVPLVTGERKREALKVMEDVARKKSSRMYVIDKD-FSVKVKSLKLHENRFDYCGE-NTFEDLVLTMNGPHQIENAGVALKTLEATG------LPLSEKAIREGLKNAKNLGRFEILEKNGKMYILDGAHNPHGAESLVRSLKLYFNGEPLSLVIGILDDKNREDILRKYTGIFERVIVTRVPSPRMKDMNSLVDMAKKFFKNVEVIEDPLEAIE----------STERATVVTGSLFLVGYVREFL-

General information: TITO was launched using: RESULT: Template: 1O5Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2359 -101871 -43.18 -255.96 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -43.18 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_1O5Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O5Z-query.scw PDB file : Tito_Scwrl_1O5Z.pdb: