Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREDTKVYDITIIGGGPVGLFTAFYGGMRQASVKIIESLPQLGGQLSALYPEKYIYDVAGFPK-IRAQELINNLKEQMAKFDQTICLEQAVESVEKQADGVFKLVTNEETHYSKTVIITAGNGAFKPRKLELENAEQYEGKNLHYF-VDDLQKFAGRRVAILGGGDSAVDWALMLEPIAKEVSIIHRRDKFRAHEHSVENLHASKVNVLTPFVPAELIGEDKIEQLVLEEVK------GDRKEILEIDDLIVNYGFVSSLGPIKNWGLDIEKNSIVVKSTM-----ETNIEGFFAAGDICTYEGKVNLIASGFGEAPTAVNNAKAYMDPKARVQPLHSTSLFENK 1TRB Chain:A ((3-314)) ----TKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEK-GGQLTTTTE---VENWPGDPNDLTGPLLMERMHEHATKFETEIIFDH-INKVDLQ-NRPFRLNGDNGEYTCDALIIATGASA---RYLGLPSEEAFKGRGVSACATSDGFFYRNQKVAVIGGGNTAVEEALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGVTGVRLRDTQNSDNIESLDVAGLFVAIGH-SPNTAIFEGQLELENGYIKVQSGIHGNATQTSIPGVFAAGDVMDHIYRQAITSAGTG--CMAALDAERYLD-----------------

General information: TITO was launched using: RESULT: Template: 1TRB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1736 31065 17.89 103.90 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 17.89 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_1TRB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TRB-query.scw PDB file : Tito_Scwrl_1TRB.pdb: