Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGFYNSGGYSGNSGYSNGFGSSFALIVVLFILLIIVGAAIFNY 3W5A Chain:C ((1-31)) ------------MERSTRELCLNFTVVLITVILIWLLVRSYQY

General information: TITO was launched using: RESULT: Template: 3W5A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 5 -618 -123.50 -19.92 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain C : 0.62 3D Compatibility (PKB) : -123.50 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.607

(partial model without unconserved sides chains): PDB file : Tito_3W5A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3W5A-query.scw PDB file : Tito_Scwrl_3W5A.pdb: