Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQISQPLSAALLMKKEDVKQLLEVRKLLEIGVASLA-AEKRTEADLERIQDALKEMGSIEA--------DGELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRETRKIWLFSKKTSVQRLYEEHERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ 3IHU Chain:A ((32-216)) ----GTFVPGQRLVETDLVAH-FGVGRNSVREALQRLAAEGIVDLQRHRGAVIRRLSL------------------QETLDVLDVAERMTGLLARAATRGSGNQPQVQ-ALR--ASVQALVAAEKAQDGETFSNARRHFYRTLLEMGDNRELRRLFPTIHMPIVHAQHRLASLR--------QMRLDDYRRIATAVLAGEPDAAEAAGAAHVKNVRGAI---------

General information: TITO was launched using: RESULT: Template: 3IHU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 644 6883 10.69 39.11 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 10.69 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_3IHU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IHU-query.scw PDB file : Tito_Scwrl_3IHU.pdb: