Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------MIVYAVNMGIVFIWSWFAKMCGGRDDSLATGYRPNKLLIW-----IPLASLVLVSGLRYRVGTDFQTYTLLYELAGDYQNVWQIFGFGTAKTATDPGFTALLWLMNFITEDPQIMYFTVAVVTYSFIMKTLADYGRPFELSVFLFLGTFHYYASFNGIRQYMVAAVLF--WAIRYIISGNWKR-----YFLIVLVSSLFHSSALIMIPVYFIVRRKAWSPAIFGLSALFLGMTFLYQK-------------FISVFVVVLENSSYSHYEKWLMTNTNGMNVIKIAVLVLPLFLAF-CYKERLRSLWPQIDIVVNLCLLGFLFGLLATKD-----VIFARFNIYFGLYQMILVPYFVRIFDEKSNALIYIAI------------------VVCYFLYSYLLM------------PVDSSVLPYRTIFSR------------------------------------------------ 5EZM Chain:A ((28-578)) SAATGWVVLFVAVALVVWFVSLDMRHLVGPDEGRYAEISREMFASGDWVTIRYNALKYFEKPPFHMWVTVVGYELFGLGEWQARLAVALSGLLGIGVSMMAARRWFGARAAAFTGLALLAAPMWSVAAHF--NTLDMTLAGVMSCVLAFMLMGQHPDASVAARRGWMVACWAAMGVAILTKGLVGIALPGLVLVVYTLVTRDWGLWRRLHLALGVVVMLVITVPWFYLVSVRNPEFPNFFFIHEHWQRRSGSVFYFLPLVIGGFLPWAGIFPKLWTAMRARFRPALMAGIWAIAIFVFFSISRSKLPGYIVPVIPALGILAGVALDRLSPRSWGKQLIGMAIVAACGLLASPVVATLNANHIPNSFYRAYAVWVAVAFVVMLLGIAVARLLLRRGVLPSVAVYAMGMYLGFTVALLGH--ETVGRPASGADIAPQIAQKLTPEMPLYGVQMLDHTLPFYLRHPLMMVGQADELTFGATVEPQRVVPDVDSFTKLWKNGQPAMAVMSPDTYLALAPTLSMYVVARDWRRVVVANVASLAGPQ

General information: TITO was launched using: RESULT: Template: 5EZM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1668 -279253 -167.42 -769.29 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -167.42 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.243

(partial model without unconserved sides chains): PDB file : Tito_5EZM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EZM-query.scw PDB file : Tito_Scwrl_5EZM.pdb: