Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEIKLKYDTVIKTLDSVKDALADVSIGAAGSNG--KNSLD------YTKKYHEREENIKTMLGDYKKAVQKNIEDTKDNVDSLKEQDEAIAVK 4LWS Chain:A ((35-124)) --QSAVDRAAMAQAAQDIEQSANAIRGMQNQ-LASAKDQLRSHWEGDASMAFEAVFNRFNEDFSRVLKALDGMHESLVQTRITYEAREEAAQQ-

General information: TITO was launched using: RESULT: Template: 4LWS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 109 11416 104.73 139.22 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 104.73 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.615

(partial model without unconserved sides chains): PDB file : Tito_4LWS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LWS-query.scw PDB file : Tito_Scwrl_4LWS.pdb: