Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKALIPKQHGAWAMLLIPFLLGMVKGGPVIWHIPLFLGWLFLYLAVYPVTLALKKKQSKPYQKWMCYYGFPTCCFLMISVFHKPPLIWVGVSLLPLFLIHMYFARRKNERALLNDVAGVLFFCSGGFASCWLGMGTLDGWAWFIFLQSALFFIGSSFYVKSVIRERKNRAFAY--WSWGYHLLLPFLSALFGAGWAFLAFIPSSLRAWFFHGRDWPVKTIGILEIVNACFFLAVMCLFITR 1P32 Chain:A ((74-282)) ---MHTDGDKAFVDFLSDEIKEERKIQKHKTLPKMSGGW---ELELNGTEAKLVRKVAG------------EKITVTFNINNSIPL-----TSTPNFVVEVIKNDDGK------------------------KALVLDCHYPEDEAESDIFSIREVSFQSTGESEWKDTNYTLNTDSLDWALYDHLMDFLADRG------VDNTFADELVELSTALEHQEYITFLEDLKSFVKSQ------

General information: TITO was launched using: RESULT: Template: 1P32.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 668 -60504 -90.57 -336.13 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -90.57 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.143

(partial model without unconserved sides chains): PDB file : Tito_1P32.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P32-query.scw PDB file : Tito_Scwrl_1P32.pdb: