Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNINIVTIGKLKEKYLKQGIEEYTKRLSAYAKIDIIELPDEKAPENLSDQDMKIIKDKEGDRILSKISPDAHVIALAIEGKMKTSEELADTIDKLATYGKSKVTFVIGGSLGLSDTVMKRADEKLSFSKMTFPHQLMRLILVEQIYRAFRINRGEPYHK 1TO0 Chain:A ((1-157)) MNINIVTIGKLKEKYLKQGIEEYTKRLSAYAKIDIIELPDEK---------MKIIKDKEGDRILSKISPDAHVIALAIEGKMKTSEELADTIDKLATYGKSKVTFVIGGSLGLSDTVMKRADEKLSFSKMTFPHQLMRLILVEQIYRAFRINRGEPY--

General information: TITO was launched using: RESULT: Template: 1TO0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 655 35118 53.62 240.53 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.98 3D Compatibility (PKB) : 53.62 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.168

(partial model without unconserved sides chains): PDB file : Tito_1TO0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TO0-query.scw PDB file : Tito_Scwrl_1TO0.pdb: