Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMSSYPRQRPRRLRSTVAMRRLVAQTSLEPRHLVLPMFVADGIDEPRPITSMPGVVQHTRDSLRRAAAAAVAAGVGGLMLFGVPRDQDKDGVGSAGIDPDGILNVALRDLAKDLGEATVLMADTCLDEFTDHGHCGVLDDRGRVDNDATVARYVELAVAQAESGAHVVGPSGMMDGQVAAIRDGLDAAGYID-VVILAYAAKFASAFYGPFREAVSSSLS-GDRRTYQQEPGNAAEALREIELDLDEGADIVMVKPAMGYLDVVAAAADVSP-VPVAAYQVSGEYAMIRAAAANNWIDERAAVLESLTGIRRAGADIVLTYWAVDAAGWLT 5HNR Chain:A ((21-327)) -----------------------TATTTLNASNLIYPIFVTDVPDDIQPITSLPGVARYGVKRLEEMLRPLVEEGLRCVLIFGVPSRVPKDERGSAADSEESPAIEAIHLLRKTFPNLLV-ACDVCLCPYTSHGHCGLLS--GAFRAEESRQRLAEVALAYAKAGCQVVAPSDMMDGRVEAIKEALMAHGLGNRVSVMSYSAKFASCFYGPFRDAAKSSPAFGDRRCYQLPPGARGLALRAVDRDVREGADMLMVKPGMPYLDIVREVKDKHPDLPLAVYHVSGEFAMLWHGAQAGAFDLKAAVLEAMTAFRRAGADIIITYYTPQLLQWL-

General information: TITO was launched using: RESULT: Template: 5HNR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -185006 for 2613 contacts (-70.8/contact) + 2D Compatibility (PS) -34700 + (NN) -25469 + (LL) 2136 1D Compatibility (HY) -24400 + (ID) 6700 Total energy: -274139.0 ( -104.91 by residue) QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_5HNR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HNR-query.scw PDB file : Tito_Scwrl_5HNR.pdb: