Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAEFKRKGGGKVKLVVGMTGATGAIFGVRLLQWLKAAGVETHLVVSPWANVTIKHETGYTL----QEVEQLATYTYSHKDQA----------AAISSGSFDTDGMIVAPCSMKSLASIRTGMADNLLTRAADVMLKERKKLVLLTRETPLNQIHLENMLALTKMGTIILPPMPAFYNRPRSLEEMVDHIVFRTLDQFGIRLPEAKRWNGIEKQKGGA 4RHE Chain:F ((11-205)) -------------KITLAITGASGASYAMRLIECLIAANYQLYILCSSAGRIVLDTEVGVKIPSSPDAASKFLTEKYQAKDQQITVFGKEQWFSPVASGSSAPKQMVVCPCSTGTMAAICHGMSDNLIERAADVVIKERGQLILMVRETPFSTLHLQNMLSLSQQGVTIMPASPGFYHKVETIEDLIDFMVGRVLDHLGIEQDIMPRW----------

General information: TITO was launched using: RESULT: Template: 4RHE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 878 -158288 -180.28 -874.52 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain F : 0.80 3D Compatibility (PKB) : -180.28 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_4RHE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RHE-query.scw PDB file : Tito_Scwrl_4RHE.pdb: