Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNINVDVKQNENDIQVNIAGEIDVYSAPVLREKLVPLAEQGADLRICLK--DVSYMDSTGLGVFVGTFKMVKKQGGSLKLENLSERLIRLFDITGLKDIIDISAKSEGGVQ 1TIL Chain:F ((4-114)) MSLAIDLEVKQDVLIVRLSGELDHHTAEELREQVTDVLENRAIRHIVLNLGQLTFMDASGLGVILGRYKQIKNVGGQMVVCAVSPAVKRLFDMSGLFKIIRVEADEQFALQ

General information: TITO was launched using: RESULT: Template: 1TIL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 472 -79031 -167.44 -725.06 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain F : 0.79 3D Compatibility (PKB) : -167.44 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_1TIL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TIL-query.scw PDB file : Tito_Scwrl_1TIL.pdb: