Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFKVFLLAASTIAVGLVELIVGGILPQIAN------DLDISIVSAGQLISVFALGYAVSGPLLLALTAKI-ERKRLYLIALFVFFLSNLVAYFSP--NFATLMVSRVLAAMSTGLIVVLSLTIAPKIVAP--EYRARAIGIIFMGFSSAIALGVPLGILISDSFGWRILFLGIGLLALISMLIISIF-FERIPAE--KMIPF-------REQ-----------------------------------------------------LKTIGNLKIAS----SHLVTMFTLAGHYTLYAYFAPFLEETLHLS----SFWVSICYFLFGISAVCGGPFGGALSDRLGSFKS------ILLVTGSFAIIMFLLPLST----------SSMIFFLPVMVIWGLLSWSLAPAQQSYLIEIAPD-SSDIQQSFNTSALQVGIALGSAIGGVVLDQ--TGTVVSTAWCGGSIVIIAVLFAFISLTRPVQTAKKSSL 4W6V Chain:A ((19-490)) ---PLSGLFLSEMWERFSFYGIRPLLILFMAATVFDGGMGLPREQASAIVGIFAGSMYLAALPGGLLADNWLGQQRAVWYGSILIALGHLSIALSAFFGNDLFFIGLVFIVLGTGLFKTCISVMVGTLYKPGDARRDGGFSLFYMGINMGSFIAPLLSGWLLRTHGWHWGFGIGGIGMLVALLIFRGFAIPAMKRYDAEVGLDSSWNKPT---GRWVTAIMAVVVVIIALISQGVIPINPVMIASLLVYVIAASVTLYFIYLFAFAKMSRKDRARLLVCFILLVSAAFFWSAFEQKPTSFNLFANDYTDRMVMGFEIPTVWFQSINALFIILLAPVFSWA---------PSSITKFVIGILCAAAGFAVMMYAAQHVLSSGGAGVSPLWLVMSILLLTLGELCLSPIGLATMTLLAPDRMRGQVMGLWFCASSLGNLAAGLIGGHVKADQLDM-LPTLFARCSIALVICAAV-----------------

General information: TITO was launched using: RESULT: Template: 4W6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1823 -333202 -182.78 -938.60 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -182.78 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.250

(partial model without unconserved sides chains): PDB file : Tito_4W6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4W6V-query.scw PDB file : Tito_Scwrl_4W6V.pdb: