Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRIILLLAVIIAAAAAGVAFYVAKDKGHEKAADVSVNTESGDELLVSITDTDLLTKYYENDKVIHEEKLTSYPAFALDQKQQVLYYTGNNE-----QNEMRLFKLDLKSHKKTMLYKGA---ESADSLFLSKDRSTIYFRLGKADESNFRIAAFDLKTKKYKNLYPAANDQDD-----------------TVSSFFYNQKNDSFALLHYSVEEDYKKTDEANEK--------GI-----DPEPTTIHFAEGR-QNKFNELKS--------LNQFISDIAVSDDDKRILFTSYTQKGTEQTASIQMLNADTKKYESIISNQKSFKLLID----AQPQFSKDGKNIYFL-----AEAKGAKKLKDETGREAKVRTIYSYSLENKTFKKVWENPNGIINSFFVIN 4WJL Chain:A ((63-345)) -------------------------------------------------------------------------ASRHSVSPDLKYVLLAYDVKQIFHYSYTASYVIYNIHTREVWELNPPEVEDSVLQYAAWGVQGQQLIYIF------ENNIYYQPDIKSSSLRLTSS--GKEEIIFNGIADWLYEEELLHSHIAHWWSPDGERLAFLMINDS-LVPTMVIPRFTGALYPKGKQYPYPKAGQMNPTIKLYVVNLYGPTHTLELMPPDSFKSREYYITMVKWVSNTKTVVRWLNRAQN---ISILTVCETTTGACSKK-YEMTSD--TWLSQQNEEPVFSRDGSKFFMTVPVKQGGRGEF-------------HHIAMFLIQSK--------------------

General information: TITO was launched using: RESULT: Template: 4WJL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 981 -83656 -85.28 -368.53 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -85.28 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_4WJL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WJL-query.scw PDB file : Tito_Scwrl_4WJL.pdb: