Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAEIKGLNEECGVFGIWGHEEAPQITYYGLHSLQHRGQEGAGIVATDGEK-LTAHKGQGLITEVFQNGELSKVKGKGAIGHVRYATAGGGGYENVQPLLFRSQNNGSLALAHNGNLVNATQLKQQL-ENQGSIFQTSSDTEVLAHL----IKRSGHFTLK-DQIKNSLS----MLKGAYAFLIMTETEMIVAL-DPNGLRPLSIGMMG-----DAYVVASETCAFDVVGATYLREVEPGEMLIINDEG-MKSERFSMNINRSICSMEYIYFSRPDSNIDGINVHSARKNLGKMLAQESA-----VEADVVTGVPDSSISAAIGYAEATGIPYELGLIKNRYVGRTFIQPSQALREQGVRMKLSAVRGVVEGKRVVMVDDSIVRGTTSRRIVTMLREAGATEVHVKISSPPIAHPCFYGIDTSTHEELIASSHSVEEIRQEIGADTLSFLSVEGLLKGIGRKYDDSNCGQCLACFTGKYPTEIYQDTVLPHVKEAVLTK 1ECJ Chain:C ((1-468)) -----------CGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFRLRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPY--GITLAHNGNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAATNRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLGFDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSARVNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRTFIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYLASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAENPDIQQFEC-SVFNGVYVTK-----------------

General information: TITO was launched using: RESULT: Template: 1ECJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2778 -264379 -95.17 -594.11 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -95.17 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_1ECJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ECJ-query.scw PDB file : Tito_Scwrl_1ECJ.pdb: