Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKSISFFVIFSILWGSLFLFSIIGSLGTTPIPLTKDSKFLMSSILPQGWGFFSKNPRDTAIGLYEAENASAKVRWPNMRADNLFGLYRYGRSQGVEMGVIY-SQVGKEQWTACKEKDL--GACKSKAKTVQLKTPAPRPLLCGSYYLTKEDIVPWSYSKYTPSSYQVKSIVKVVISCSKT 2LMZ Chain:A ((1-42)) ---------------------------------------------------------------------------------------------------IPYCGQTGAECYSWCIKQDLSKDWCCDFVKDIRMNPPADKCP----------------------------------------

General information: TITO was launched using: RESULT: Template: 2LMZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 117 -5072 -43.35 -130.04 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : -43.35 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_2LMZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LMZ-query.scw PDB file : Tito_Scwrl_2LMZ.pdb: