Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNSANTTAPSLLSAYGLNSYTSSNSGSVTKAAESTETAVADSASNKHEANQIRSGDFSIDSAIKKAADKYGVDEKLIRAVIKQESGFNAKAVSGAGAMGLMQLMPSTA-----SSLGVSNP------LDPQQNVEGGTKYLKQMLDKYDGNVS-------MALAAYNAGPGNVDRYGGIPPFQETQNYVKKITSVYYA 4CFP Chain:B ((178-290)) ---------------------------------------------------------------VRQASRKYGVDESLILAIMQTQSSFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAMLNNVYLGGIDNPTSRRYAVITAYNGGAGSVLR-----------------------

General information: TITO was launched using: RESULT: Template: 4CFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 355 -46128 -129.94 -485.56 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -129.94 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_4CFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CFP-query.scw PDB file : Tito_Scwrl_4CFP.pdb: