Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAFILSAAAAVGLFTFGGVQQASAKELSCQPVVTVKTGNTVQNMSLNDAVKKLHINTNIKTLNAANEKEMKQLLQKHAKQSNVKVQDVQKTETAKPAQKTTEKAAADQNTASKAPATAEKTNTTTSAPSSVSAYEKKVVELTNAERQKQ-----------------GLKPLQIDETLSKSARAKSQDMKDKNYFDHQSPTYGSPFDMMKSFGISYKTAGENIAKGQ-----KTPEEVVKAWMN---------------------SEGHRKNILNPNFTHIGVGYVESGSIWTQQFIGK 2VZN Chain:A ((38-181)) -----------------------------------------------------------------------------------------------------------------------------------FTDAEKDAIVNKHNELRQRVASGKEMRGTNGPQPPAVKMPNLTWDPELATIAQRWANQCTF----EHDACRN-----------VERFAVGQNIAATSSSGNKSTPNEMILLWYNEVKDFDNRWISSFPSDDNILMKVGHYTQIVWAKTTKIGCGRIMF-----------

General information: TITO was launched using: RESULT: Template: 2VZN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 341 -11920 -34.96 -119.20 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -34.96 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_2VZN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VZN-query.scw PDB file : Tito_Scwrl_2VZN.pdb: