Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTYHPFSLTTPSTLMIQDWAQTNQNNKEVIAGFTTKNGGVSQKPFESLNTGLHVHDKDADVVKNREYIADMFNTDLQSWVFADQTHDNRVQKVTQRDRGKGAREYHTALKATDGIYTNEKNVFLALCFADCVPLFFYDPVKSLVGVAHAGWKGTVKQIGREMVKQWTEKEGSNPSDIYAVIGPSISGACYTVDDRVMDAVRALPVSADLAANQTAKAQYQLDLKELNRLILMDSGLASEQISVSGLCTESEPSLFYSHRRDQGKTGRMMSFIGMKEA 1U05 Chain:B ((2-240)) ------------SKLIVPQWPLP----KGVAACSSTRIGGVSLPPYDSLNLGAHCGDNPDHVEENRKRLFAAGNLPSKP-VWLEQVHGKDVLKLTGEP---------YASKRADASYSNTPGTVCAVMTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADK----PENILAWLGPAIGPRAFEVGAEVREAFMAVDAKASAAFIQHGD-KYLADIYQLARQRLANVGV--EQIFGGDRCTYTENETFFSYRRDK-TTGRMASFI-----

General information: TITO was launched using: RESULT: Template: 1U05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1451 -148034 -102.02 -619.39 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -102.02 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.316

(partial model without unconserved sides chains): PDB file : Tito_1U05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U05-query.scw PDB file : Tito_Scwrl_1U05.pdb: