Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKKFVPFAVFLFLFFVSFEMMENPHALDYIGAMKKDTVTVTASKDPLYEELLQKAPEYEVKPQNARIDKVWKSIPGYNGLKVNIEQSYKKMKQHGKFREKDLVYSQVKPSVHLESLQPEPIYKGNPDKPMVAFLINVAWGNEYLEKMLPILQKHQVK-ATFFLEGNWVRNNVQLAKKIAKDGHEIGNHSYNHPDMSKLTTGRISEQLDKTNEQIEQTIGVKP-KWFAPPSGSFRKAVIDIAAEKQMGTVMWTVDTIDWQKPAPSVLQTRVLSKIHNGAMILMHPTDP---TAESLEALITQIKDKGYALGTVTELMDETRLLK 4M1B Chain:B ((47-246)) -----------------------------------------------------------------------------------------------------------------------KVIYKGDTSKKQVAFTFDISWGDKKAIPILDTLKERDIKNATFFLSAAWAERHPDVVERIIKDGHEIGSMGYNYTSYTSLETNEIRRDLLRAQDVFTK-LGVKQIKLLRPPSGDFNKATLKIAESLGYTVVHWSNNSNDWKNPGVNKIVSTVSNNLKGGDIVLLHASDSALQTNKALPLLLQKLKSDGYEQISVSQLISNT----

General information: TITO was launched using: RESULT: Template: 4M1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1044 -106174 -101.70 -544.48 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -101.70 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.547

(partial model without unconserved sides chains): PDB file : Tito_4M1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M1B-query.scw PDB file : Tito_Scwrl_4M1B.pdb: