Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNKRRNRQIVVAVNGGKAVKAIFLFIVSLIVIFVLSGVLTSLRPELRPSSDSFYGIAEELPGDVFAHLLQMENHYFASDLSQTDSSFHLSRLSLKLATSINLEDPRSFLGRELPGFAQFDTEILLAGQGTDYTNMPAESPPPSKVMEEEREANLAEIEKQQTQSDNAQKDPPKQTTGDKKVVFIYHTHNTESYLPLLKGETDPDMARHSKANVTLVGDMFGQALES-QGIGATVNKTDIQSKLNKKGLNYARSYDESRPVVKDALASNKNLQYIIDIHRDSRRKKDTTATIKGKSYARVAFVVGKKSKNFEENYKIASELHKLMEKKYPGLSTGVFSKGS-PGDN-GVYNQDLTDRALLLEFGGVDNNLEE------LQRAANAAADVFSEMYWDAEKVNAASGETKKQ 3CZX Chain:A ((30-179)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AQDMRNIVASILRNDYGLTVKTDGTGKGN----------MPLRD---AVKLI----RGSDVAIEFHTNAAANK----TATGIE---A-LST-------PKNKRWCQVLGKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYA-QAGGIVFEPFFISNDTDLALFKTTKWGICRAIADAIAMELGA--------------

General information: TITO was launched using: RESULT: Template: 3CZX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 653 28516 43.67 202.24 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 43.67 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_3CZX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CZX-query.scw PDB file : Tito_Scwrl_3CZX.pdb: