Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERISWNQYFMAQSHLLALRSTCPRLSVGATIVRDKRMIAGGYNGSIAGGVHCADEGCLMIDDHCARTIHAEMNAILQCSKFGVPTDGAEIYVTHYPCIQCCKSIIQAGIKTVYYAEDYKTHPYAQELFEQAGVTVEQVELDEMIVDLKNREKLSFVAGLIGKLADAGLAEEELKKIHEQANTLFTSYV 2HVW Chain:C ((38-181)) --RLSWQDYFMANAELISKRSTCNRAYVGAVLVKNNRIIATGYNGGVADTDNCDDVGHEMEDGHCIRTVHAEMNALIQCAKEGISANNTEIYVTHFPCINCTKALLQAGVKKITYNTAYRIHPFAIELMTQK--EVEYVQHDVPRVKL-----------------------------------------

General information: TITO was launched using: RESULT: Template: 2HVW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 721 -99337 -137.78 -689.84 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -137.78 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_2HVW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HVW-query.scw PDB file : Tito_Scwrl_2HVW.pdb: