Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENFIGSHMIYTYENGWEYEIYIKNDHTIDYRIHSGMVAGRWVRDQEVNIVKLTEGVYKVSWTEPTGTDVSLNFMPNEKRMHGIIFFPKWVHEHPEITVCYQNDHIDLMKESREKYETYPKYVVPEFAEITFLKNEGVDNEEVISKAPYEGMTDDIRAGRL 2W2B Chain:B ((24-183)) LDDFLGTHFIYTFDNGWEYEWYAKNDHTVDYRIHGGMVAGRWVTDQKADIVMLTEGIYKISWTEPTGTDVALDFMPNEKKLHGTIFFPKWVEEHPEITVTYQNEHIDLMEQSREKYATYPKLVVPEFANITYMGDAGQNNEDVISEAPYKEMPNDIRNGK-

General information: TITO was launched using: RESULT: Template: 2W2B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 772 -64780 -83.91 -404.87 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.95 3D Compatibility (PKB) : -83.91 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_2W2B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W2B-query.scw PDB file : Tito_Scwrl_2W2B.pdb: