Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSNFSQPVVQLCYEDVNGSCIETPYSPGSRVILYTAFSFGSLLAVFGNLLVMTSVLHFKQLHSPTNFLIASLACADFLVGVTVMLFSMVRTVESCWYFGAKFCTLHSCCDVAFCYSSVLHLCFICIDRYIVVTDPLVYATKFTVSVSGICISVSWILPLTYSGAVFYTGVNDDGLEELVSALNCV---GGCQIIVSQGWVLIDFLL-FFIPTLVMIILYSKIFLIAKQQAIKIETTSSKVESSSESYKIRVAKRERKAAKTLGVTVLAFVISWLPYTVDILIDAFMGFLTPAYIYEICCWSAYYNSAMNPLIYALFYPWFRKAIKLILSGDVLKASSSTISLFLE 4AMJ Chain:B ((23-300)) ------------------------------------------LLIVAGNVLVIAAIGSTQRLQTLTNLFITSLACADLVVGLLVVPFGATLVVRGTWLWGSFLCELWTSLDVLCVTASIETLCVIAIDRYLAITSPFRYQSLMTRARAKVIICTVWAISALVS---FLPIMMHWWRDEDPQALKCYQDPGCCDFVTNRAYAIASSIISFYIPLLIMIFVALRVYREAKEQIRKIDRASKRKTSRV------MLMREHKALKTLGIIMGVFTLCWLPFFLVNIVNVFNRDLVPDWLFVAFNWLGYANSAMNPIIYCR-SPDFRKAFKRLLA----------------

General information: TITO was launched using: RESULT: Template: 4AMJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -236951 for 2012 contacts (-117.8/contact) + 2D Compatibility (PS) -27345 + (NN) -748 + (LL) 3892 1D Compatibility (HY) -24400 + (ID) 4750 Total energy: -290302.0 ( -144.29 by residue) QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_4AMJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4AMJ-query.scw PDB file : Tito_Scwrl_4AMJ.pdb: