Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLMKALLGLVDSTGE---SGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGL----YKERGLFKRLSKTDWEKVSRVIDQVGLRGFENR-PINALSGGQFQRMLMARCLVQEADYIFLDEPFVGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQAFTRENLSAAYGDAILLGQGD 4HLU Chain:C ((17-230)) ------VSFRYNGDYVLKDVNAEFETGKIYVVVGKNGSGKTTLLKILAGLLAAAGEIFLDGSPADPFLLRKNVGYVFQNPSSQI-IGATVEEDVAFSLEIMGLDESEMRKRIKK--------VLELVGLSGLAAADPLN-LSGGQKQRLAIASMLARDTRFLALDEPVSMLDPPSQREIFQVLESLKNEGKGIILVTHELEYLDDMDFILHISNGTIDFCGSWEEFVEREFDDVEIPFKWKLWKKC

General information: TITO was launched using: RESULT: Template: 4HLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -166812 for 1600 contacts (-104.3/contact) + 2D Compatibility (PS) -22490 + (NN) -9751 + (LL) 1524 1D Compatibility (HY) -17600 + (ID) 3750 Total energy: -218879.0 ( -136.80 by residue) QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_4HLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HLU-query.scw PDB file : Tito_Scwrl_4HLU.pdb: