Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MFLFTNGKVLWGAVIAAFILSIVFYPFLPTQMPIHYDVANSPDLTVNKLAGTVMLPVLMVVFAWARKIN--WQFVFAVYILLICHI------VVLCLAL- 4HAR Chain:A ((25-122)) EACVTSWLWSEGE---GAV---FYRVDLHFTNLGTP-PLDEDGRWDPALMYNPCGPEPPAHVVRAYNQPAGDVRGVWGKGERTYAEQDFRVGGTRWHRLLRMPVR

General information: TITO was launched using: RESULT: Template: 4HAR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 185 -40754 -220.29 -485.16 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -220.29 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.088

(partial model without unconserved sides chains): PDB file : Tito_4HAR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HAR-query.scw PDB file : Tito_Scwrl_4HAR.pdb: