Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKIKYLYQLLLSHISILILAFVIIISLFSHFVKEFAYQNKVEELTSYAVQIANEFQSGQVDMRRLYPYQDILSTRKTQFIIFNEEQQPYFLPEGFHHPPREQLKKSEWNKLKKGQTVTIRADGRFDDEVSLVAQPIFVQNEFKGAVLLISPISGVEQMVNQVNLYMFYAVISTLVITILVSWLLSKFHVKRIQKLREATDKVASGDYDIHLENSYGDEIGVLASDFNIMAKKLKQSRDEIERLEKRRRQFIADVSHELKTPLTTINGLVEGLNSHTIPEDKKDKCF-SLISEETKRMLRLVKENLDYEKIRSQQITLNKLDVPLIEVFEIVKEHLQQQAEEKQNKL---MIQVE---DHVIVHADYDRFIQILVNITKNSIQFTQNGDI-WLRGMEGYKETIIEIEDTGIGIPKEDIEHIWERFYKADISRTNTAYGEYGLGLSIVRQLVEMHQGTVEIKSEEGKGTKFIIRLPLTAKQQ 4U7O Chain:B ((37-261)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QFVSNVSHELRTPLTSLRSYIEALSDGAWKDPEVAPGFLKVTQEETDRMIRMINELLSLS------TRVDMELVNINEMFNYVLDRFDMILKKDDNPAKYYTIKREFTKRDLWVEIDTDKFTQVLDNIMNNAIKYSPDGGVVTCRLLETHNQVIISISDQGLGIPRADLGHVFDRFFRVDKAR----QGGTGLGLAISKEVVQMLGGRIWVDSVEGKGSTFYISLP------

General information: TITO was launched using: RESULT: Template: 4U7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 901 -72632 -80.61 -349.19 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -80.61 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_4U7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U7O-query.scw PDB file : Tito_Scwrl_4U7O.pdb: