Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------MIKFSVILGMIRCSLTHI-TT--KNTVNALKRMIYPKQKPSFFHEFKVLYKLLKKFCIKGIMIKNIRSCMGYFL------------------------------------------------------------------------------------------------------------------ 3TZG Chain:A ((28-272)) DTYVTKVTDLTGEEEQVLKLEYDRDGKIIKYGDTPVRYEGDQITIGQMNKLCNVTFQIGKGKARESRARCMLKVGEEVYEADKQTVYDYKGDTIFINSDYRATSDYRF-LKKVQGKYVFDQLGRLKEVMTVFTEANDSVSSCHTYYNYDNNINYQANLNLQAYVIDYDGVDSFFYFLLNLGQLRNRTALPNDIGYCMNHGLSTYNVHANYRLDDENPVRIEVLYNYTKLLSRIDLSYNPL

General information: TITO was launched using: RESULT: Template: 3TZG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 219 -13050 -59.59 -186.42 target 2D structure prediction score : 0.20 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -59.59 2D Compatibility (Sec. Struct. Predict.) : 0.20 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.104

(partial model without unconserved sides chains): PDB file : Tito_3TZG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TZG-query.scw PDB file : Tito_Scwrl_3TZG.pdb: