Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEALTFESKYALLYLGGGLLAMIYGLITFFMAFPAFTSRGNVIFTIKTGPSGEIFLRKR---SVLFSEVKRLYMGRHQYSLKGIFFEDIIIEKTDGKIVRIPTWNIITNPLFF---EAVERYILPHLNEEAQNNWISQFTEVQRKAYLKEFENHPKL 4Z2N Chain:A ((813-905)) ----------------------------------------STCLLQPT--SSALVNATEWPPFVVTLDEVELIHFERVQFH-LKNF--DMVIVYKDYSK-KVTMINAIPVASLDPIKEWLNSCDLKYTEGVQSLNWTKI-------------------

General information: TITO was launched using: RESULT: Template: 4Z2N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 295 -42651 -144.58 -490.24 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -144.58 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_4Z2N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z2N-query.scw PDB file : Tito_Scwrl_4Z2N.pdb: