Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIAIAAITATSILALSACSSGDKEVIAKTDAGDVTKGELYTNMKKTAGASVLTQLVQEKVLDKKYKVSDKEIDNKLKEYKTQLGDQYTALEKQYGKDYLKEQVKYELLTQKAAKDNIKVTDADIKEYWEGLKGKIRASHILVADK--------------KTAEEVE-------KKLKKGEK-FEDLAKEYSTDSSASKGGDLGWFAKEGQMDETFSKAAFKLKTGEVSDPVKTQYGYHIIKKTEERGKYDDMKKELKSEVLEQKLNDNAAVQEAVQKVMKKADIEVKDKDLKDTFNTSSTSNSTSSSSSNSK 4TYO Chain:B ((9-123)) ----------------------------------------------------------------------------------------------------------------------------------QGEPARVRCSHLLVKHSQSRRPSSWRQEQITRTQEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSR-GQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TYO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 396 -23960 -60.51 -257.63 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -60.51 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.674

(partial model without unconserved sides chains): PDB file : Tito_4TYO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TYO-query.scw PDB file : Tito_Scwrl_4TYO.pdb: