Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVYITYGTADFLKTIVQKHPSENILLMQGQENAILIHETNGDTVFQAPHAYEVIDQVGEIKHPGFAVLNNIAVTQEGRPLFENRFKNRAGKVENEPGFEAIRVLRPLDSDTYVILTLWETESAFQDWQQSGSYKEAHKKRDTSAGIDTTSIFSRPSYVTTYFAVE 4FVC Chain:F ((1-165)) MKVYITYGTADFLKTIVKKHPSENILLMQGQENAILIHETSGDTVFQAPHAYEVIDQVGEIKHPGFAVLANIAVTQEGRPLFENKFKNRAGKVENEPGFEAIRVLRPLDSDTYVILTLWETERAFQDWQQSDSY-----------GIDTTSIFSRPSYVTTYFAV-

General information: TITO was launched using: RESULT: Template: 4FVC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 738 -100385 -136.02 -651.85 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain F : 0.98 3D Compatibility (PKB) : -136.02 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.661

(partial model without unconserved sides chains): PDB file : Tito_4FVC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FVC-query.scw PDB file : Tito_Scwrl_4FVC.pdb: