Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAGILGVGRYIPEKVLTNHDLEKMVETSDEWIRTRTGIEERRIAADDVFSSHMAVAAAKNALEQAEVAAEDLDMILVATVTPDQSFPTVSCMIQEQLGAKKACAMDISAACAGFMYGVVTGKQFIESGTYKHVLVVGVEKLSSITDWEDRNTAVLFGDGAGAAVVGPVSDDRGILSFELGADGTGGQHLY-----------LNEKRHTIMNGREVFKFAVRQMGESCVNVIEKAGLSKEDVDFLIPHQANIRIMEAARERLELPVEKMSKTVHKYGNTSAASIPISLVEELEAGKIKDGDVVVMVGFGGGLTWGAIAIRWGR 1UB7 Chain:D ((2-320)) --SGILALGAYVPERVMTNADFEAYLDTSDEWIVTRTGIKERRVAAEDEYTSDLAFKAVEDLLRRHPGALEGVDAVIVATNTPDALFPDTAALVQARFGLK-AFAYDLLAGCPGWIYALAQAHALVEAGLAQKVLAVGAEALSKIIDWNDRATAVLFGDGGGAAVVGKVREGYGFRSFVLGADGTGAKELYHACVAPRLPDGTSMKNRLYMNGREVFKFAVRVMNTATLEAIEKAGLTPEDIRLFVPHQANLRIIDAARERLGLPWERVAVNVDRYGNTSTASIPLALKEAVDAGRIREGDHVLLVSFGAGLTWAAAVLTWG-

General information: TITO was launched using: RESULT: Template: 1UB7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2029 -236138 -116.38 -766.68 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.87 3D Compatibility (PKB) : -116.38 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_1UB7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UB7-query.scw PDB file : Tito_Scwrl_1UB7.pdb: