Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYGIVLFPSKK-LQDLANSYRKRYD-----PSYSLIPP---HLTLRASFECAEEKAD-------QLVSHLRNIAKESHPLVLKMTKYSSFAPVNNVIYIKAEPT---EELKTLNEKLYTGVLA--G--EQEYNFVPHVTVGQNLSDDEH---------SDVLGQLKMQEVSHEEIVDRFHLLYQLE----NGSWTVYETFLLGRGE 3J4Q Chain:A ((90-291)) PNYFLSIPITNKKITAGIKVLQNSILRQDNRLTKAMVGDGSFHITLLVMQLLNEDEVNIGTDALLELKPFVEEIL-EGKHLTLPFHGIGTFQG--QVGFVKLADGDHVSALLEIAETAKRTFQEKGILAGESRTFKPHLTFMKLSKAPMLWKKGVRKIEPGLYEQFIDHR-FGEEILYQIDLCSMLKKKQSNGYYHCESSIVIGEK-

General information: TITO was launched using: RESULT: Template: 3J4Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 701 -107383 -153.19 -646.89 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -153.19 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_3J4Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J4Q-query.scw PDB file : Tito_Scwrl_3J4Q.pdb: