Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYYTTAETPLGELIIAEEEDRITRLFL--SQEDWVDWKETVQNTEHKETPNLAEAKQQLQEYFAGERKTFSLPLSQKGTP-FQQK------VWQALERIPYGESRSYADIAAAVGSPKAVRAVGQANKRNDLPIFVPCHRVIGKNSALTGYAGSKTEIKAFLLNIERISYKEK 1T39 Chain:B ((22-184)) ----TTLDSPLGKLELSGCEQGLHEIKLLG-----------------PEP--LMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGG-LAVKEWLLAHE-------

General information: TITO was launched using: RESULT: Template: 1T39.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 584 -71622 -122.64 -534.49 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -122.64 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_1T39.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T39-query.scw PDB file : Tito_Scwrl_1T39.pdb: