Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYTVTLNPSVDYIVHVEDFTVGGLNRSSYDTKYPGGKGINVSRLLKRHHVASKALGFVGGFTGEYIKTFLREENLETAFSEVKGDTRINVK-LKTGDETEINGQGPTISDEDFKAFLEQFQSLQEG-DIVVLAGSIPSSLPHDTYEKIAEACKQQNARVVLDISGEALLKATE--MKPFLMKPNHHELGEMFGTAITSVEEAVPYGKKLVEQ----GAEHVIVSMAGDGALLFTNEAVYFANVPKGKLVNSVGAGDSVVAGFLAGISKQLPLEEAFRLGVTSGSATAFSEELGTEEFVQQLLPEVKVTRL 2JGV Chain:D ((22-284)) -ILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTRVLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEILEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPVILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPL---DESLESLKQAVSQPLFEGIEWIIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAI--------------------------------------------

General information: TITO was launched using: RESULT: Template: 2JGV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1298 -129490 -99.76 -507.80 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : -99.76 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_2JGV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JGV-query.scw PDB file : Tito_Scwrl_2JGV.pdb: