Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKKTFVKKAMLTTAAMTSAALLTFGPDAASAKTPVDNTAVQLQHQASTNEDLNTFIDILNQCIYEQDGVYYFDSEKAVELGMTKEEAQVIATLWESTSEFFSIVSQCVYLEDGNYKFDTEKAVELGFTEKEALALEQFFSAVSLKIHILQAAIVL---QDDVYSYDKDAALQAGATPLQADVYEKLFSALSQEQLAAIYDMIHPQA------ 1MPG Chain:A ((100-282)) AARPGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPT--------PQRLAAADPQALKALGMPLKRAEALIHLANAALE---------------------GTLPMTIPGDVEQAMKTLQTFPGIGRWTANYFALRGWQAKDVFLPD-DYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEGWQPDEA

General information: TITO was launched using: RESULT: Template: 1MPG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 698 -82332 -117.95 -475.91 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -117.95 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_1MPG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MPG-query.scw PDB file : Tito_Scwrl_1MPG.pdb: