Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQHKTVLLRETVDGLNIKPDGTYVDCTLGGAGHSTYLLQQLSEKGRLIAFDQDDTALQHAKEVLSDYKGQLILIKSNFRYLKEYLNEQGVTEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSATLSAKEVVNEWRYEDLVRIFFKYGEEKFSKQIARKIEEARMKSPIQTTGQLVDLIKDAIPAPARRSGGHPAKRVFQAIRIAVNDELRVFEEALEQAIEVLKPGGRVSVITFHSLEDRICKTTFKEKSSLPELPPGLPVIPEEFEPELKLITRKPITASQEELEENNRARSAKLRIAEKRK 1WG8 Chain:B ((5-282)) --THVPVLYQEALDLLAVRPGGVYVDATLGGAGHARGILER---GGRVIGLDQDPEAVARAKGL---HLPGLTVVQGNFRHLKRHLAALGVERVDGILADLGVSSFHLDDPSRGFSYQKEGPLDMRMGLEGP-TAKEVVNRLPLEALARLLRELGEEPQAYRIARAIVAAREKAPIETTTQLAEIVRKAV---GFRRAGHPARKTFQALRIYVNDELNALKEFLEQAAEVLAPGGRLVVIAFHSLEDRVVKRFLRESG-------------------LKVLTKKPLVPSEKEAAQNPRARSAKLRAAEK--

General information: TITO was launched using: RESULT: Template: 1WG8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1527 -140577 -92.06 -505.67 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -92.06 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_1WG8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WG8-query.scw PDB file : Tito_Scwrl_1WG8.pdb: