Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILQLDNVSLKRNGKWILKDIHWKVEEKENWVLYGLNGAGKTALLNMLCSYYFPTSGEMQVLGHEFGKTELGEKLRRKIGLVSA--ALQQKLYPADSAFEIALSGAYASIGLYETPSKETREKAIGLLEDLGAIEYADRRYETLSQGEKQRALIARALMADPELLILDEPVTGLDFIAREKLLDTITYIANKENAPSILYVTHHAEEILPVFDKALLLKQGEVFGSGEIKEMLTDQILSAFFDTPIHVLWNQDRPFLTRAEPITNA 4HZI Chain:B ((34-277)) -LLSLEKISYKPTGKTILDSVSFEIKTNEHCVLLGRNGAGKSTLVNLIYGMIWATSGTIRLFQETYGEIAI-QDLRKRIGILDSSQ---RKLTVKDT----ILTGL--------DPSPEEETKTLQILKDSDLLSKKDQLYNTLSSGEKKKILFLRSIVNEPDFLIMDEPCSSLDLTAREDFLGFLKEYHSKKKFTS-LYITHRPEEIPDFYSKAVLLKEGKVIHFGPIEECFTEKNLEDLYDIPLQV------------------

General information: TITO was launched using: RESULT: Template: 4HZI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1095 -147667 -134.86 -647.66 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -134.86 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4HZI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HZI-query.scw PDB file : Tito_Scwrl_4HZI.pdb: