Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKLDLQPTEKLFIPFITAGDPVPEVSIELAKSLQKAGATALELGVAYSDPLADGPVIQRASKRALDQGMNIVKAIELGGEMKKNGVNIPIILFTYYNPVLQLNKEYFFALLQENHIDGLLVPDLPLEESNSLQEECKSHEVTYISLVAPT-SESRLKTIIEQAEGFVYCVSSLGVTGVRNEFNSSVYPFIRTVKNLSTVPVAVGFGISNREQVIKMNEI-SDGVVVGSALVRKIEELKDRLISAETRNQALQEFEDYAMAFSGLYSLK 1RD5 Chain:B ((20-254)) ------------FIPYITAGDPDLATTAEALRLLDGCGADVIELGVPCSDP--DGPIIQASVARALASGTTMDAVLEMLREVTPE-LSCPVVLLSYYKPIMFRS----LAKMKEAGVHGLIVPDLPYVAAHSLWSEAKNNNLELVLLTTPAIPEDRMKEITKASEGFVYLVSV-----------PRVESLIQEVKKVTNKPVAVGFGISKPEHVKQIAQWGADGVIIGSAMVRQLGE-------AASPKQGLRRLEEYA----------

General information: TITO was launched using: RESULT: Template: 1RD5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1153 -174108 -151.00 -791.40 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -151.00 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.606

(partial model without unconserved sides chains): PDB file : Tito_1RD5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RD5-query.scw PDB file : Tito_Scwrl_1RD5.pdb: