Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYNKEEKIKSLNRMQYEVTQNNGTEPPFQNEYWDHKEEGLYVDIVSGKPLFTSKDKFDSQCGWPSFTKPIEEE-VEEKLDTSHGMIRTEVRSRTADSHLGHVFNDGPGP-NGLRYCINSAALRFVPKHKLKEEGYESYLHLFNK 3E0M Chain:C ((179-313)) ----------SLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPLFFAKDKFASGCGWPSFSRPLSKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPRELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK

General information: TITO was launched using: RESULT: Template: 3E0M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 679 11805 17.39 88.76 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain C : 0.84 3D Compatibility (PKB) : 17.39 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_3E0M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E0M-query.scw PDB file : Tito_Scwrl_3E0M.pdb: