Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQITDISQLKEAIKQYHSEGKSIGFVPTMGFLHEGHLTLADKARQENDAVIMSIFVNPAQFGPNEDFEAYPRDIERDAALAENAGVDILFTPDAHDMYPGEKNVTIHVERRTDVLCGRSREGHFDGVAIVLTKLFNLVKPTRAYFGLKDAQQVAVVDGLISDFFMDIELVPVDTVREEDGLAKSSRNVYLTAEERKEAPKLYRALQTSAELVQAGERDPEAVIKAAKDIIET-TSGTIDYVELYSYPELEPVNEIAGKMILAVAVAFSKARLIDNIIIDIREMERI 2X3F Chain:B ((5-282)) -KLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRESVSTNDITVVSVFVNPLQFGPNEDF-AYPRQIDKDLELVSEVGADIVFHPAVEDMYPGELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQMVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGERQSKVIIDKVTQYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLIDNIII--------

General information: TITO was launched using: RESULT: Template: 2X3F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1394 -148444 -106.49 -537.84 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -106.49 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.626

(partial model without unconserved sides chains): PDB file : Tito_2X3F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X3F-query.scw PDB file : Tito_Scwrl_2X3F.pdb: