Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIFKKAVFVIMISFLIATVNVNTAHAAIDVSAKSAIIIDGASGRVLYAKDEHQKRRIASITKIMTAVLA---IESGKMDQT--VTVSANAVRTEGSAIYLTEGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLDGFVYMMNQKAEQLGMKNTRFQNPHGLDDHENHYSTAYDMAILTKYAMKLKDYQKISGTKIYKAETMESVWKN---KNKLLTMLYPYSTGG-KTGYTKLAKRTLVSTASKDGIDLIAVTINDPNDW---DDHMKMFNYVFEHYQTYLIAKKG-DIPK---LKGTFYESKAFIKRDITYLLTEEEKENVKINTTLLKPKKAWEKDASKIPDIVGHMEIMFNDATIAKVPIYYENERHQKPKKQFFETFKSIFLNAAGGAKWSI 4K91 Chain:B ((8-330)) --------------------------APPQLAAKSYVLMDGESGQVLVENNGDQRLPPASLTKLMTAYIATKEIEAGRIGENDLVTVSEHAWRTGGSRMFIKVGSQVSVSDLLHGIIIQSGNDASVALAEHIAGSEDAFADMMNTTAQKLGLTNSHFMDATGLPN-PDHYSSARDMAVLARAII----YGEPSHYAIYAQK--EFLWNNIKQPNRNLLLWRDKTVDGLKTGHTDEAGYCLVASAVRDGQRMIAVVFGTNSEQARAAETQKLLTYGFRFFESRNFYKKGTELTKGLVWKGSEHEVKAGLAEDLTMTLPRGQMQKLQA-SMVLEPQLMAPIQQGQ---VIGKVEVKLDDKVI--------------------------------------

General information: TITO was launched using: RESULT: Template: 4K91.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1725 83069 48.16 270.58 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 48.16 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4K91.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K91-query.scw PDB file : Tito_Scwrl_4K91.pdb: