Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKQTVWLLTMLSLVVVLSVYYIMSPESKNAVQMQSEKSASDSGEVATEKAPAKQDTKEKSGTETEKGKEDGTKGTKDSSADKETSAEASEKGTVVTETADDDLFTTYRLDLEDARSKEREELNAIVSSDDATAKEKSEAYDKMTALSEVEGTEKQLETLIKTQGYEDALVNAEGDKINITVKSDKHSKSKATAIIDLVAKEIKTMKDVAVTFEPSK 1M5N Chain:Q ((67-94)) -------------------------------------------------------YLTQETNKVETYKEQPLKTPGKKKKGKP---------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1M5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Q - contact count / total energy / energy per contact / energy per residue : 23 7168 311.65 256.00 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain Q : 0.56 3D Compatibility (PKB) : 311.65 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.733

(partial model without unconserved sides chains): PDB file : Tito_1M5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M5N-query.scw PDB file : Tito_Scwrl_1M5N.pdb: