Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKSILKIAAAACDDKRAEDILALDMEGISLVADYFLICHGNSDKQVQAIAREIKDQADENGIQVKKMEGFDEARWVLVDLGDVVVHVFHKDERSYYNLEKLWGDAPLADLDFGMNQ 2O5A Chain:B ((3-112)) --NQELLQLAVNAVDDKKAEQVVALNMKGISL--DFFLICHGNSEKQVQAIAHELKKVAQEQGIEIKRLEGYEQARWVLIDLGDVVVHVFHKDERAYYNLEKLW---PTVEL------

General information: TITO was launched using: RESULT: Template: 2O5A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 505 -91205 -180.60 -868.62 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -180.60 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.718

(partial model without unconserved sides chains): PDB file : Tito_2O5A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O5A-query.scw PDB file : Tito_Scwrl_2O5A.pdb: